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. 2007 May 21;177(4):731–742. doi: 10.1083/jcb.200609175

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Increase of the cytoplasmic IP₃ concentrations induced by CD59 clusters, as observed by the epifluorescence imaging of the PH domain (from PLCδ1)–GFP expressed in T24 cells. Typical results are shown. CD59 clustering was induced by the addition of IgG-gold particles or the natural ligand, C8, whereas Fab-gold is a non–cross-linking control. Pretreatment of cells is summarized in the legend to Fig. 2 and Materials and methods.