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. 2006 Jul 31;174(3):339–348. doi: 10.1083/jcb.200511109

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Dynamic shuttling of Tbx5 between nuclear and cytoplasmic compartments. COS-7 cells cotransfected with EGFP-Tbx5 and LMP4. (A) Photobleaching of cytoplasmic EGFP-Tbx5. Graph displays quantitative fluorescent data for a representative cell. (B) Photobleaching of nuclear EGFP-Tbx5. Graph displays representative quantitative fluorescent data. Bleaching of whole cell displayed no fluorescence recovery within the displayed time frame (Fig. S3, available at http://www.jcb.org/cgi/content/full/jcb.200511109/DC1). All FRAP assays were performed with a minimum of three cells.