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. 2006 Jul 31;174(3):349–358. doi: 10.1083/jcb.200512103

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Characterization of the gw mutation and localization of the GW protein. (A) gw ¹ is caused by a nonsense mutation of the tryptophan codon at position 967 to stop. (B) The gw ¹ mutation causes the loss of an NcoI restriction site and allowed rapid embryo genotyping by PCR. (C) Mutations were confirmed by DNA sequencing. (D) A polyclonal antibody raised against the N-terminal region of GW recognizes a 160-kD band representing the endogenous protein. (E) The anti-GW antibody also recognizes a 100-kD truncated form of GW in gw¹/gw¹ embryos that is not present in wild-type embryos.