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. 2006 Jul 31;174(3):349–358. doi: 10.1083/jcb.200512103

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Colocalization of GW with markers associated with GWBs/PBs in Drosophila S2 cells. (A) A C-terminal fusion of RFP to PCM localized to discrete cytoplasmic foci. Several of these (arrows) colocalized with a GFP-GW fusion protein. (B) Another human GWB component, LSm4, localized to the nucleus (middle), but some signal was also detected in cytoplasmic foci (arrows). Some, but not all, Drosophila GWBs colocalized with the LSm4 foci. (C) AGO2, a RISC component, also colocalized with some cytoplasmic Drosophila GWBs (arrows). Notably, the cytoplasmic bodies containing GFP-GW and RFP-AGO2 were consistently larger than those containing only GFP-GW. (D–F) Protein fusions between RFP and the three major human GW182 family proteins transfected into Drosophila S2 cells were found in the same structures as Drosophila GW. The expression of human GW182 could not be detected without a coincident RNAi knockdown of endogenous GW. Bars, 5 μM.