Figure 8.

Lack of functional GW protein leads to nuclear breakdown caused by defects in mitosis. (A) In wild-type embryos of the same age, a regular array of nuclei, each with a pair of centrosomes, can been seen immediately below the embryo cortex. (B) In homozygous gw ¹ embryos 90 min AED stained with anticentrosomin (Cnn, red) antibody and antitubulin (blue), severe defects are observed after NC10. Fewer nuclei (PicoGreen) are seen, and the majority of these have improperly localized centrosomes. Large, brightly staining DNA aggregates are also seen (arrowheads). Maximum projection of 125 slices that are 10 μm deep. Bars, 5 μM.