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. 2006 Jul 31;174(3):459–471. doi: 10.1083/jcb.200508170

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Internalization of TeNT H_C is dependent on a functional clathrin machinery. 25 h after microinjection of the AP-2 μ2^T156A and tTA plasmids (d–f), or 26 h after microinjection of a plasmid encoding AP180-C (g–i), MNs were incubated with 20 nM b-TeNT H_C and 10 ng/ml Alexa Fluor 555–CTB for 30 min, MESNA-treated, fixed, stained for internal biotin and the epitope tags of the microinjected constructs, and imaged. (a–c) Uninjected MNs were imaged as a control. Bar, 10 μm.