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. 2006 Aug 14;174(4):593–604. doi: 10.1083/jcb.200602080

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Copyright © 2006, The Rockefeller University Press

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Figure 9. — VEC does not codistribute with VEGFR-2 in internal compartments. VEC-positive cells were treated with VEGF for 10 min, double stained for VEGFR-2 and VEC, and analyzed by confocal microscopy. Besides junctional staining, VEC did not show any obvious vesicular pattern. VEGFR-2 and VEC appeared to codistribute only at cell–cell contacts and not in intracellular compartments. The bottom panel on the right (2.6-fold magnification of the boxed areas) shows the colocalization of VEGFR-2 and VEC (yellow) set upon the VEC background (gray). This was obtained through the colocalization plugin of ImageJ (see Materials and methods for details). Arrows point to the junctional colocalization of VEGFR-2 and VEC. Bars, 10 μm.