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. 2006 Aug 28;174(5):625–630. doi: 10.1083/jcb.200606051

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Depletion of PCM-1 and pericentrin prevents DNA replication. (A) Immunofluorescence of MRC-5 fibroblasts treated with control RNA or siRNA against PCM-1 and pericentrin, respectively. Bar, 10 μm. Graphs depict the percentages of cells lacking PCM-1 or pericentrin expression after treatment with control or silencing RNAs. Cells were scored as negative if they lacked centrosomal staining of PCM-1 or pericentrin, only displaying diffuse background fluorescence. (B) Percentages of cells incorporating BrdU after treatment with control RNA or silencing RNA against PCM-1 or pericentrin. Data were obtained from cell cultures that were double labeled for BrdU and PCM-1 or pericentrin. Among cell cultures treated with silencing RNA, only cells that were visibly depleted of PCM-1 or pericentrin were scored. Error bars indicate SD.