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. 2006 Aug 28;174(5):625–630. doi: 10.1083/jcb.200606051

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Cell cycle arrest after PCM-1 depletion depends on the checkpoint protein p53. (A) Immunoblots of MRC-5 cells treated with control RNA or siRNAs against PCM-1, p53, or both PCM-1 and p53 combined. Blots were probed with antibodies against p53, PCM-1, or α-tubulin. (B) Graph depicts BrdU incorporation in p53−/− H1299 cells treated with control RNA or siRNA against PCM-1. Error bars indicate SD. (C) Immunoblots of H1299 and MRC-5 cells treated with control RNA or siRNA against PCM-1 (PCM1d) for 72 h. Blots were probed with antibodies against PCM-1, p53, PCNA, MCM3, pRb, or α-tubulin.