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. 2006 Aug 28;174(5):665–675. doi: 10.1083/jcb.200601062

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — Mutants in the MPS3 SUN domain and in MPS2 exhibit karyogamy defects. Cells from log phase cultures of the indicated mutants were mated at the semipermissive temperature of 30°C for 5 h, and then mating mixtures were stained with DAPI and the position of nuclei in zygotes was observed by fluorescence microscopy. Numbers represent the mean percentage of zygotes with a single mass of DNA (Kar+), two separated masses of DNA (congression−), and two masses of DNA separated by <2 μm (fusion−). 100 zygotes in four independent mating experiments were analyzed.