Table II.

GP values of immunostained adherent or detached MEFs

Adherent MEFs	WT MEFs	Cav1−/− MEFs
Cav1	0.382 ± 0.070 (n = 27)a
pYCav1	0.459 ± 0.076 (n = 24)a
pFAK	0.422 ± 0.058 (n = 24)b	0.297 ± 0.080 (n = 28)b
CTxB	0.434 ± 0.069 (n = 11)	0.442 ± 0.099 (n = 10)
Detached MEFs
CTxB (1 min)	0.368 ± 0.061 (n = 13)α β	0.370 ± 0.032 (n = 12)
CTxB (15 min)	0.306 ± 0.060 (n = 12)c	0.365 ± 0.050 (n = 12)c
CTxB (30 min)	0.270 ± 0.058 (n = 16)d α	0.353 ± 0.040 (n = 12)d
CTxB (120 min)	0.212 ± 0.086 (n = 14)e β	0.330 ± 0.037 (n = 12)e

MEFs on FN-coated coverslip for 4 h (adherent) or in suspension for 1–120 min (detached) were labeled and imaged as described in Materials and methods. Mean GP values ± the SD (from n images) were calculated for pixels stained positively for Cav1, pYCav1, pFAK, and CTxB. Pairs of footnotes denote statistically significant differences between the two values sharing the same footnote.

^a,bP < 0.001.

^cP < 0.05.

^d,eP < 0.001. c–e compare WT MEFs to Cav1^−/− MEFs.

^αP < 0.05.

^βP < 0.001. α and β compare the kinetics of CTxB-labeled domains in WT MEFs. No significant differences were found between adherent cells and cells immediately after detachment (1 min) for both WT and Cav1^−/− MEFs, and no differences were found in the kinetics of CTxB-labeled domains in Cav1^−/− MEFs.