Figure 3.
cki-2(RNAi) causes defects in the elimination of the maternal centrosome. (A–C) Early wild-type one-cell embryo (A; prepronuclear migration stage) or cki-2cs embryos that express GFP–γ-tubulin to visualize centrosomes (B and C). (D–F) Early one-cell embryos (prepronuclear migration stage) from rrf-3 (D) or rrf-3; cki-2(RNAi) (E and F) adult hermaphrodites stained with anti–SPD-2 antibody. The arrows indicate polar bodies stained with DAPI (anterior). Asterisks mark centrosomes (maternal [m] and paternal [p]). The white rectangular box in A shows the paternal centrosome that could not be observed in the same focal plane. The rectangular boxed regions in B and D–F were magnified to show greater detail.