Table II.
Supernumerary centrosomes are present in the one-cell embryo of cki-2cs animals
| Genotype | Embryonic lethality | Supernumerary centrosomea |
|---|---|---|
| % | % | |
| rrf-3 | 23.0 ± 1.2 (n = 374) | 0 (n = 76) |
| rrf-3; cki-1 (RNAi) | 94.7 (n = 570) | 0 (n = 40) |
| rrf-3; cki-2 (RNAi) | 27.5 ± 3.7 (n = 734) | 4.5 (n = 111) |
| N2; [fem-1∷cki-2C]b | 26.9 (n = 466) | 13.5 (n = 133) |
| TH27; [fem-1∷cki-2C]b | 29.1 (n = 1257) | 6.7 (n = 60) |
One-cell embryos obtained from the cki-2 cosuppression transgene-bearing animals (GFP+) were examined to score the frequency (%) of the supernumerary centrosomes. For RNAi of cki-1 or -2, each dsRNA was injected into rrf-3 hermaphrodites as described (see Materials and methods), and the frequency (%) of both the embryonic lethality and the supernumerary centrosomes was scored. The embryonic lethality was presented as the percentage of unhatched embryos from total progeny obtained from the RNAi-treated mothers.
Embryos were stained with anti–SPD-2 or γ-tubulin∷GFP, and the results are presented as the percentage of the total number of one-cell stage embryos examined. All one-cell embryos examined were at or before the first cell division.
The frequency of the supernumerary centrosome defect was determined in the most penetrant cosuppressed lines (line #1 of N2; [fem-1∷cki-2C] and TH27; [fem-1∷cki-2C]) for comparison.