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. 2006 Sep 11;174(6):803–813. doi: 10.1083/jcb.200604014

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Ultrastructural localization of ER in Jurkat cells before and after store depletion. Jurkat cells were transiently transfected with HRP-ER plasmid and processed for HRP cytochemistry and EM. (A and B) In resting cells with full Ca²⁺ stores, HRP-ER was located in the nuclear envelope and numerous cytoplasmic tubules (arrowheads), as well as in tubules closely associated with the plasma membrane (arrows). Large, round electron-dense structures (asterisks) were present in HRP-expressing cells as well as in untransfected cells, indicating that they are probably DAB-reactive peroxisomes or lysosomes. (C and D) In cells treated with 1 μM TG for 15 min to deplete stores, the overall distribution of HRP-ER was similar to that of resting cells, with dense reaction product present in both cytosolic and plasma membrane–associated tubules. The area outlined by the box in C is shown at higher magnification in D. n, nucleus; pm, plasma membrane. Bars: (A and C) 2 μm; (B and D) 200 nm.