Table I.
MT turnover during metaphase and preanaphase B in wild-type and stable GST–cyclin B–injected spindles in Drosophila embryos
| Near pole
|
Equator
|
||||
|---|---|---|---|---|---|
| Embryo | Mitotic stage | t1/2 | Percentage of recovery | t1/2 | Percentage of recovery |
| s | s | ||||
| Wild type | Preanaphase B | 8.6 ± 2.3 (n = 10) | 81.0 ± 14.3 (n = 10) | 5.8 ± 0.9 (n = 11) | 89.5 ± 6.8 (n = 11) |
| Wild type | Anaphase B | 2.8 ± 0.5 (n = 20) | 45.8 ± 11.2 (n = 20) | 5.3 ± 1.3 (n = 20) | 85.8 ± 8.8 (n = 20) |
| Stable cyclin B injected | Preanaphase B arrested |
7.8 ± 2.2 (n = 23) | 85.0 ± 8.13 (n = 23) | 6.57 ± 0.9 (n = 20) | 88.0 ± 8.6 (n = 20) |
The t1/2 and percentage of recovery values for the pole and equator shown in the table are from experiments where the spindles were simultaneously bleached at the two regions.