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Cytosolic antioxidant EUK-143 prevents hypoxic stabilization of HIF-1α protein. HIF-1α protein levels of whole cell lysates from WT cybrids (A) and ΔCyt b cybrids (B) preincubated with 10 μM EUK-134 for 2 h and then subjected to 21% O2 (N), 1.5% O2 (H), or 1 mM DMOG (D) for 4 h. Representative blot from three independent experiments.
