Figure 6.

Hypoxic HIF-1α protein stability is attenuated by pharmacological inhibitors of the Q_o but not Q_i site in WT cells. (A) HIF-1α protein levels of whole cell lysates from WT cybrids incubated with 1 μM stigmatellin and subjected to 21% O₂ (N), 1.5% O₂ (H), or 1 mM DMOG (D) for 4 h. Representative blot from three independent experiments. (B) HIF-1α protein levels of whole cell lysates from WT cybrids incubated with 1 μM antimycin A and subjected to 21% O₂ (N), 1.5% O₂ (H), or 1 mM DMOG (D) for 4 h. Representative blot from three independent experiments.