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. 2007 Jun 18;177(6):1029–1036. doi: 10.1083/jcb.200609074

Figure 9.

Figure 9.

Reduced cytochrome c is not sufficient to stabilize the HIF-1α protein. (A) HIF-1α protein levels of 143B and A549 cells subjected to 21% O2 (N), 1.5% O2 (H), and 1 mM DMOG (D) for 2 h. Representative blot from three independent experiments. (B) HIF-1α protein levels of 143Bρ0 and A549ρ0 whole cell lysates <21% O2 (N) or 1.5% O2 (H) for 2 h after a 15-min pulse of 100 μM of the cytochrome c–reducing agent TMPD and 400 μM ascorbate (T) or 1 mM DMOG (D). Representative blot from three independent experiments. (C) Cytochrome c and p66Shc protein levels in 143Bρ0 and A549ρ0 whole cell lysates. Representative blot from three independent experiments.