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. 2007 Jun 18;177(6):1133–1143. doi: 10.1083/jcb.200612068

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Biochemical screening for the target Rabs of specific TBC domain proteins. To determine the specific activity toward a range of GTPases, 0.5 pmol RN-tre, RabGAP-5, TBC1D10B, and TBC1D17 were tested against 100 pmol of the Rab GTPases indicated using the procedures described in Materials and methods. All reactions were performed for 60 min. The basal GTP hydrolysis seen with a buffer control was subtracted for each GTPase, and the mean GTP hydrolysis in picomoles/hour is plotted in the figures. Error bars represent SD.