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. 2007 Jun 18;177(6):1133–1143. doi: 10.1083/jcb.200612068

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Functional verification of the targets of RN-tre and RabGAP-5. (A–D) HeLa cells were transfected with siRNA duplexes to deplete all three isoforms of Rab5 (A and B) or Rab43 (C and D). (A and D) EGF uptake assays were performed, and the extent of EGF transport at 30 min is shown in the figure. EGF is in green, and EEA1 is in red. (B and C) Shiga toxin uptake assays were performed, and the extent of STxB transport to the Golgi at 30 min is shown. STxB is in red, and GM130 is in green. (E) HeLa cells transfected with GFP-tagged Rab43 (green) were fixed after 24 h and stained with antibodies to the Golgi marker TGN46 or the early endosome marker EEA1 (red). DNA is stained blue in all panels. Bar, 10 μm.