Skip to main content
. 2007 Jun 18;177(6):1133–1143. doi: 10.1083/jcb.200612068

Table I.

Effects of human TBC domain–containing proteins on Shiga toxin transport

Rab GAP
Wild-type TBC domain
Catalytically inactive TBC domain
Target
Rab		 STxB GAP
localization		 EGF Golgi STxB GAP
localization		 EGF
EVI-5 35 Vesicular Cytosolic Early
endosomesa 		Intact Golgi Tubules,
cytosolic		 ND
RN-tre
    (USP6NL)		 41 Cell surface
vesicular		 Cell surface Early
endosomesa 		Fragmented Golgi Cell surface ND
RabGAP-5
    (RUTBC3)		 5A-C Golgi Cytosolic Cell surface Intact Golgib Cytosolic Early
endosomes
TBC1D10A ND Vesicular
Golgi		 Cell surface Early
endosomesa 		Intact Golgi Cell surface ND
TBC1D10B 22a/31 Vesicular Cell surface Early
endosomes		 Intact Golgi Cell surface ND
TBC1D10C ND Vesicular Filopodia,
cell surface		 Early
endosomes		 Intact Golgi Cell surface ND
TBC1D11
    (GAPCenA)		 4>11 Golgi Cytosolic Early
endosomes		 Intact ND Cytosolic ND
TBC1D14 ND Recycling
endosomes		 Recycling
endosomes		 Early
endosomes		 Fragmented Recycling
endosomes		 Recycling
endosomes		 ND
TBC1D17 21 Vesicular Cytosolic Early
endosomes		 Intact Golgi Cytosolic ND
TBC1D22A 33A-Bc Golgi
fragments		 Cytosolic,
nuclear		 Early
endosomes		 Fragmented Golgi Cytosolic/
nuclear		 ND
TBC1D22B 33A-Bc Golgi
fragments		 Cytosolic,
nuclear		 Early
endosomes		 Fragmented Golgi Cytosolic/
nuclear		 ND

HeLa cells were transfected for 24 h with GFP-tagged wild-type or catalytically inactive Rab GAPs. These cells were then used for SSTxB or EGF uptake assays, and the uptake of STxB and EGF were scored at 1 h or 30 min, respectively. A 0-min time point was also taken to verify that both STxB and EGF were bound to the cell surface with equal efficiency for all conditions. Localization of the GFP-tagged Rab GAPs was also scored.

a

Some highly expressing cells failed to bind EGF at the zero time point, indicating a potential defect in the trafficking of EGF receptor to the cell surface. However, the majority of cells bound and took up EGF as normal.

b

The RabGAP-5 GAP domain was used for this experiment.

c

TBC1D22 family GAPs have previously been shown to act on Rab33 (Pan et al., 2006).