Figure 4.

Ran accumulation in the nucleus and at the NE. (A–C) Nuclear accumulation of fluorescent Ran. Shown is the average of 101 confocal frames collected between 600 and 800 s after reaction initiation. Images were acquired under the same conditions and displayed with the same scaling. Unless noted above the images, concentrations were the same as in Fig. 1. Bar, 10 μm. (D) Time course of the NE accumulation of Ran under the conditions of A (red), B (blue), and C (green). The vertical dashed lines (red) indicate the time interval during which single-molecule transport experiments were conducted (unless otherwise noted).