Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Sep 25;174(7):951–961. doi: 10.1083/jcb.200605053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — In vivo single-molecule nuclear import. (A) Bright-field image of two HeLa cells before microinjection. Bar, 10 μm. (B) Epifluorescence image of the two cells in A ∼3 min after cytoplasmic microinjection of NLS-2xGFP(4C). The Alexa 555–labeled cargo localized to the nuclei after microinjection. (C) Histogram of NE interaction times after microinjection of NLS-2xGFP(4C). Data were pooled from cargo tagged with Alexa 555 and cargo labeled with Alexa 647 (see Table S1, available at http://www.jcb.org/cgi/content/full/jcb.200605053/DC1). τ = 7.8 ± 0.4 ms (n = 307); import efficiency = 51 ± 5% (n = 115). (D) Histogram of NE interaction times after microinjection of Alexa 647 dextran (10 kD). τ = 1.8 ± 0.1 ms (n = 374); import efficiency = 50 ± 4% (n = 141).