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. 2006 Sep 25;174(7):985–996. doi: 10.1083/jcb.200604069

Figure 6.

Figure 6.

Effect of ATP depletion on cell size and the actin cytoskeleton. (A) Impact of ATP depletion on cell size. ATP levels and cell size were determined after 48-h treatment of NIH control cells with increasing doses of oligomycin. Similar results were obtained for HeLa cells (not depicted). (B) Impact of ATP depletion on the actin cytoskeleton. NIH control cells were cultured in the absence or presence of 10 nM oligomycin for 2 d, and then stained to visualize actin and chromatin. Note the reduction of stress fibers, cell size, and adhesion surface. (C) Impact of ATP on wound healing. The NIH control monolayer was wounded, and the size of the gap was evaluated at different times. n = 3. (D) Impact of complex I and II inhibition on cell size. Control NIH cells were treated with 10 nM rotenone or 1 mM TTFA for 48 h, and the cellular ATP level and cell size were measured. (E and F) Effect of the depletion of the mitochondrial PiC on cell size. The phosphate carrier was depleted using two distinct siRNAs, and the abundance of the mRNA transcript and the protein levels (E) or cell size (F) were assessed 2 d after transfection. Asterisks indicate significant effects on cell size (n = 3) in phosphate carrier–depleted cells, as compared with control transfectants. Error bars represent the mean ± the SD.