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. 2006 Sep 25;174(7):997–1007. doi: 10.1083/jcb.200605004

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — The early fusion of lysosomes with phagosomes is impaired in Syt VII^−/− BMMs. BMMs were exposed to opsonized zymosan red for 10 min, fixed, and stained with anti-Lamp1 mAbs (A and B) or phalloidin (C and D). (A) Examples of the morphological criteria used to score phagosomes for fusion or no fusion. (B) Percentage of phagosomes that fused with Lamp1-containing lysosomes. (C) Images of phalloidin-stained recently formed phagosomes in Syt VII^+/+ or VI^−/− BMMs. The arrows point to phagosomes containing zymosan red surrounded by phalloidin-stained polymerized actin. (D) Percentage of phalloidin-positive phagosomes. (B and D) The data represent the mean ± SD (error bars) of triplicates. Bars, 5 μm.