Figure 5.

Syt VII–YFP is rapidly recruited to phagocytic cups and to nascent phagosomes. Selected frames from a confocal time-lapse video of a BMM transduced with Syt VII–YFP and exposed to 10 IgG-opsonized zymosan particles/cell (Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200605004/DC1). The images show the rapid recruitment of Syt VII–YFP to sites of particle attachment and to expanding phagocytic cups (arrows). At later time points, Syt VII–YFP recruitment is also detected on areas of plasma membrane ruffling, which were associated (frames at 1 min 12 s and at 3 min 34 s) or not associated (frames at 9 min 32 s and at 10 min 43 s) with particle uptake (arrowheads). Top panels, YFP channel; bottom panels, differential interference contrast. Bar, 10 μm.