Figure 8.

Syt VII–YFP-containing intracellular compartments contribute to phagosome formation. (A) Representative confocal images of anti-CD11b–AlexaFluor546 antibodies or Syt VII–YFP on the plasma membrane, phagosomes (arrowheads), and plasma membrane ruffles (arrows) of transduced BMMs. Bars, 5 μm. (B) Quantitation of the relative fluorescence intensity of Syt VII–YFP on 206 phagosomes. Each bar represents the number of phagosomes with a phagosome/plasma membrane fluorescence ratio within the intervals indicated. Black bars, phagosomes (81% of the total) in which Syt VII–YFP was enriched in relation to the plasma membrane (ratio of >1). White bars, phagosomes showing a ratio of <1. Data were normalized to the fluorescence intensity of the plasma membrane of the same cell.