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. 2006 Sep 25;174(7):1023–1033. doi: 10.1083/jcb.200604136

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — PfROM4 is a merozoite plasma membrane protein. (A) Western blot showing extracts of parental 3D7 and 3D7HAROM4_synth probed with mAb 3F10 (anti-HA) and polyclonal anti-AMA1 (loading control). The size of the anti-HA–reactive species detected only in 3D7HAROM4_synth is close to the predicted mass (90.4 kD) of HA-tagged PfROM4. (B) IFA of mature nonsegmented (top) and fully segmented (bottom) schizonts of 3D7HAROM4_synth, dual labeled with mAb 3F10 (anti-HA; green) and mAb 1E1 (anti-MSP1; red). The DAPI signal (blue) is not included in merged images for clarity. Bar, 5 μm.