Table I.

Summary of the kinetic parameters of recombinant C. elegans kinesin-II and sea urchin kinesin-1

Substrate or inhibitor	Recombinant C. elegans kinesin-II	Sea urchin kinesin-1
Mg-ATP	Km = 0.28 ± 0.05 mM Vmax = 0.26 ± 0.01 μm/s	Km = 0.063 ± 0.034 mM Vmax = 0.56 ± 0.10 μm/s
Mg-GTP	Km = 5.5 mM Vmax = 0.058 μm/s	Km = 1.9 ± 0.8 mM Vmax = 0.43 ± 0.08 μm/s
Mg-ADP	Competitive inhibitor Ki = 0.042 mM	Competitive inhibitor Ki = 0.156 ± 0.052 mM
Mg-ATP–γ-Sa	Competitive inhibitor Ki = 0.31 mM	Competitive inhibitor Ki = 0.014 ± 0.003 mM
Pi	Competitive inhibitor Ki = 4.4 mM	Unknown
Mg-AMPPCP	Inhibitor - not a competitive inhibitor	Inhibitor - not a competitive inhibitor
Mg-AMPPNP	Inhibitor - not a competitive inhibitor	Inhibitor - not a competitive inhibitor

^aMg-ATP–γ-S can be used as an alternative substrate by recombinant C. elegans kinesin-II. The velocity is 0.0100 ± 0.0015 μm/s at 2 mM Mg-ATP–γ-S.