Table II.

MT gliding velocities in the competitive motility assays with wild-type OSM-3 and OSM-3–G444E mutant versus kinesin-II

Parameter	Value for each assay
Kinesin-II and OSM-3
Kinesin-II (μM)	0.00	0.29	0.56	1.00	1.67	2.50	3.33	4.00	4.44	4.71	5.00
OSM-3 (μM)	5.00	4.71	4.44	4.00	3.33	2.50	1.67	1.00	0.56	0.29	0.00
Mole fraction of OSM-3 (%)	100	94	89	80	67	50	33	20	11	6	0
Mean velocity (μm/s)	1.075	0.998	0.906	0.705	0.615	0.540	0.476	0.415	0.347	0.348	0.343
Standard deviation (μm/s)	0.148	0.083	0.069	0.052	0.047	0.025	0.030	0.051	0.039	0.036	0.052
Count of MTs	55	63	57	74	59	74	63	58	53	49	30
Kinesin-II and OSM-3–G444E
Kinesin-II (μM)	0.00	0.29	0.56	1.00	1.67	2.50	3.33	4.00	4.44	4.71	5.00
OSM-3–G444E (μM)	5.00	4.71	4.44	4.00	3.33	2.50	1.67	1.00	0.56	0.29	0.00
Mole fraction of OSM-3–G444E (%)	100	94	89	80	67	50	33	20	11	6	0
Mean velocity (μm/s)	0.973	0.995	0.869	0.754	0.684	0.603	0.564	0.537	0.493	0.490	0.488
Standard deviation (μm/s)	0.162	0.180	0.159	0.204	0.077	0.096	0.086	0.052	0.078	0.076	0.043
Count of MTs	55	66	66	68	73	71	61	66	71	50	67