Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Sep 25;174(7):1107–1117. doi: 10.1083/jcb.200606056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Effects of interdomain hinge flexibility on flow-enhanced tethering. (A and B) Tether rates of microspheres of indicated radii bearing either L-selectin or L-selectinN138G perfused in media of indicated viscosities were plotted against the product , where r is the microsphere radius and is the wall shear rate. The microspheres were coated with 750 molecules μm⁻² of either L-selectin or L-selectinN138G, except in one case in B, where they were coated with 1,500 molecules μm⁻² of L-selectinN138G (blue squares). The flow chamber floor in A was coated with PSGL-1 at either 120 (red triangles and green diamonds) or 240 (blue squares) molecules μm⁻². The flow chamber floor in B was coated with a constant density of 6-sulfo-sLe^x. The data in A and B represent the mean ± the SD from three experiments. (C) Optimal (peak locations of the p _ad/(m _r m _l r) vs. curves) versus microsphere diffusivity k _B T/(6πμr) data for tethering to PSGL-1 (open circles; Yago et al., 2006) were replotted to provide calibration. The diffusivities of 3-μm radius microspheres bearing L-selectin (red triangle) or L-selectinN138G (green diamond) were plotted for comparison. (D) Maximum p _ad/(m _r m _l r) versus molecular diffusivity k _B T/(6πμl) data for tethering to PSGL-1 (open circles; unpublished data) were replotted to provide calibration, which assumed the characteristic length for molecular diffusion as l = 100 nm. The same l value was used for the L-selectin datum (red triangle), which matched the calibration curve well. The l value for L-selectinN138G is predicted to be smaller. Using the measured maximum p _ad/(m _r m _l r) value, the L-selectinN138G datum point (green diamond) was located at the intercept of y = [p _ad/(m _r m _l r)] _max (green dashed horizontal line) and the extrapolation of the calibration curve (red line). The increased molecular diffusivity for L-selectinN138G could be calculated from the x-axis value of this datum point (green dashed vertical line).

Inline graphic — Effects of interdomain hinge flexibility on flow-enhanced tethering. (A and B) Tether rates of microspheres of indicated radii bearing either L-selectin or L-selectinN138G perfused in media of indicated viscosities were plotted against the product , where r is the microsphere radius and is the wall shear rate. The microspheres were coated with 750 molecules μm⁻² of either L-selectin or L-selectinN138G, except in one case in B, where they were coated with 1,500 molecules μm⁻² of L-selectinN138G (blue squares). The flow chamber floor in A was coated with PSGL-1 at either 120 (red triangles and green diamonds) or 240 (blue squares) molecules μm⁻². The flow chamber floor in B was coated with a constant density of 6-sulfo-sLe^x. The data in A and B represent the mean ± the SD from three experiments. (C) Optimal (peak locations of the p _ad/(m _r m _l r) vs. curves) versus microsphere diffusivity k _B T/(6πμr) data for tethering to PSGL-1 (open circles; Yago et al., 2006) were replotted to provide calibration. The diffusivities of 3-μm radius microspheres bearing L-selectin (red triangle) or L-selectinN138G (green diamond) were plotted for comparison. (D) Maximum p _ad/(m _r m _l r) versus molecular diffusivity k _B T/(6πμl) data for tethering to PSGL-1 (open circles; unpublished data) were replotted to provide calibration, which assumed the characteristic length for molecular diffusion as l = 100 nm. The same l value was used for the L-selectin datum (red triangle), which matched the calibration curve well. The l value for L-selectinN138G is predicted to be smaller. Using the measured maximum p _ad/(m _r m _l r) value, the L-selectinN138G datum point (green diamond) was located at the intercept of y = [p _ad/(m _r m _l r)] _max (green dashed horizontal line) and the extrapolation of the calibration curve (red line). The increased molecular diffusivity for L-selectinN138G could be calculated from the x-axis value of this datum point (green dashed vertical line).