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. 2006 Sep 25;174(7):1107–1117. doi: 10.1083/jcb.200606056

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Effects of interdomain hinge flexibility on flow-enhanced rolling. Microspheres bearing matched densities of L-selectin or L-selectinN138G were perfused through a flow chamber containing immobilized PSGL-1 (A, C, and E) or 6-sulfo-sLe^x (B, D, and F). Rolling parameters are presented as mean rolling velocities (A and B), mean stop time (C and D), or fractional stop time (E and F). The data in A and B represent the mean ± the SD from five experiments. The data in C–F represent analyses of thousands of rolling steps collected from 10–15 microspheres rolling for 1 s at each wall shear-stress for each pair of selectin–ligand interactions.