Figure 7.

Quantification of neutrophil–microsphere aggregation in a flow field. Mixtures of neutrophils labeled with the red dye PKH26 and microspheres labeled with the green dye FITC were perfused through a flow chamber coated with HSA, as in Fig. 6. After exiting the flow chamber, the suspensions were fixed and analyzed by flow cytometry or by fluorescence microscopy. (A and D) Mixtures of neutrophils and L-selectin microspheres. (B and E) Mixtures of neutrophils and L-selectinN138G microspheres. (C and F) Mixtures of neutrophils and L-selectinN138G microspheres perfused in the presence of the anti–L-selectin mAb DREG-56. (A–C) Flow cytometry of ungated samples. The percentage of particles labeled with both dyes is listed in the top right quadrant. (D–F) Representative fluorescence micrographs. The data are representative of three independent experiments.