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. 2007 Jul 2;178(1):71–84. doi: 10.1083/jcb.200611064

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Copyright © 2007, The Rockefeller University Press

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Figure 8. — Effect of MARCH5^H43W on mitochondrial fusion rates in Mfn2KO cells. Mitochondrial fusion in Mfn2KO MEFs (A and D), Mfn2KO MEFs ectopically expressing Mfn2 (B and D), or MARCH5^H43W-CFP (C and D) was analyzed using mito-PAGFP dilution based assay. Details of the cell areas containing photoactivated mito-PAGFP (red squares) are shown below the respective images. The images of cells before photoactivation were obtained with the higher detector gain. The dilution rates of mito-PAGFP over the time of the experiment were quantified (D). Data was normalized, with the mito-PAGFP fluorescence at 30 s after photoactivation used as 100%. Data represent averages ± SD of 62 (Mfn2KO cells), 53 (Mfn2KO cells expressing MARCH5^H43W), and 32 (Mfn2KO cells ectopically expressing Mfn2) ROI measurements (D).