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. 2007 Jul 2;178(1):71–84. doi: 10.1083/jcb.200611064

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Copyright © 2007, The Rockefeller University Press

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Figure 9. — Ectopic expression of Drp1 reverses MARCH5 mutation-induced mitochondrial morphology defects. HeLa cells were transiently transfected with MARCH5-YFP, MARCH5^H43W-YFP (A; green), and MARCH5^C65S,C68S-YFP (B; green) in the presence of cotransfection with Drp1 and then immunostained with anti-Tom20 polyclonal antibodies (A and B; blue) and with anti-Drp1 mAbs (A and B; red). Asterisk represents the transfected cell in the field. Cells were scored for the morphology of mitochondria. Data represent averages ± SD of four transfections with 100 cells counted each time (C).