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. 2007 Jul 2;178(1):85–92. doi: 10.1083/jcb.200703206

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Copyright © 2007, The Rockefeller University Press

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Figure 1. — Mutations in CBP block ecdysone-triggered programmed cell death of larval salivary glands. (A–D) Living animals assayed 24 h APF with a GFP reporter expressed in larval salivary glands. (A) Salivary glands are no longer detectable in control pupae (w; UAS-GFP; SG-GAL4) but persist in CBP mutants (CBP^G0350; UAS-GFP/+; SG-GAL4/+) (B) or salivary glands in which CBP is inactivated by RNAi (w; UAS-CBP-RNAi/ UAS-GFP; SG-GAL4/+) (C) or in which diap1 is overexpressed (w; UAS-diap1/UAS-GFP; SG-GAL4/+) (D). (E and F) Larval salivary glands dissected at 0 or 13.5 h APF from controls or 0-, 13.5-, or 16-h CBP^G0350 animals (G–I). (J and K) Control and CBP^G0350 mutant (L–N) salivary glands stained for the cleaved active form of caspase-3.