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. 2007 Jul 2;178(1):85–92. doi: 10.1083/jcb.200703206

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Competence for rpr-mediated cell death occurs after the mid-third instar transition. Salivary glands were dissected from heat-treated third instar larvae (L3) at either 20–24 h after the L2–L3 molt (A, C, and E) or 34–38 h after the molt (B, D, and F) and stained with antibodies directed against cleaved active caspase-3. Heat-treated control (w) L3 (A and B) and hs-rpr transformants at 20–24 h after the molt (C) display no staining for active caspase-3, whereas salivary glands at 34–38 h after the molt are susceptible to hs-rpr–mediated cell death (D). This switch in competence to respond to rpr, however, fails to occur in CBP mutant glands (CBP^G0350; hs-rpr) (E and F).