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. 2007 Jul 2;178(1):93–105. doi: 10.1083/jcb.200703099

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Preventing protein synthesis fails to induce apoptosis. (A) Pathways of glutamine metabolism. (B) I^ER-MYC cells were incubated in the presence of OHT (MYC^ON) for 24 h. The media was then changed to that deficient in the indicated amino acids and the cells were incubated for 19 h before analyzing their viability. An average of two experiments is shown. (C) I^ER-MYC were incubated as in B except rapamycin was added as indicated 18 h before analyzing the rate of apoptosis (top panel) and phosphorylation of S6 (bottom panels). One of two independent experiments is shown.