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. 2007 Jul 2;178(1):93–105. doi: 10.1083/jcb.200703099

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Depleting glutathione fails to induce apoptosis. (A) Biosynthesis of glutathione. (B) I^{ER-MYC–C9DN} were incubated with OHT (MYC^ON) or ethanol (MYC^OFF) for 24 h, and then transferred to the medium depleted of glutamine or supplemented with 100 μM BSO. Cells were collected at the indicated time to measure reduced glutathione by flow cytometry (Materials and methods). (C) I^ER-MYC cells were treated as in A except the cells were collected at 24 h of incubation in the indicated media to determine the rate of apoptosis. Shown are the averages of two independent experiments.