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. 2007 Jul 2;178(1):93–105. doi: 10.1083/jcb.200703099

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Glutamine deprivation does not increase p53 concentration. I^ER-MYC cells were incubated in the presence of OHT (MYC^ON) or ethanol (MYC^OFF) for 24 h. The cells were then incubated either in the medium that was deficient in glutamine for 24 h, or for 36 h in the complete medium supplemented with DON (A), for 24 h supplemented with 1 mM MMPR (B), or for 18 h supplemented with 100 μM etoposide (C) before determining the rate of apoptosis (graphs) and the concentration of p53 by immunoblotting. The averages of two (A, B) or three (C) independent experiments are shown. Error bars in C are SDs.