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. 2007 Jul 16;178(2):209–218. doi: 10.1083/jcb.200612031

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Copyright © 2007, The Rockefeller University Press

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Figure 5. — Effect of heterochromatin in HML and HMR on γ-H2AX spreading. (A) A DSB (arrowhead) was generated at ∼7 kb from HML (gray box). The γ-H2AX ChIP signal was examined by quantitative PCR. The increase in γ-H2AX after HO induction (left) was calculated by normalizing the γ-H2AX ChIP signal at 1 h after HO induction (gray squares) with that before HO induction (black squares; right). The position of the telomere is marked with a black box. (B) The HMR sequences, including its own silencers (gray box), was introduced ∼41 kb from the left end of chromosome III, in which HML was replaced by LEU2 (white box). A DSB (arrowhead) led to increased γ-H2AX ∼50 kb to the right in the absence (gray squares) or presence (black squares) of the ectopic HMR sequence, but there is much less modification over HMR. (C) A DSB was generated ∼7 kb to the right of HMLα-inc (gray box). The level of γ-H2AX in a SIR3 (black diamonds) or sir3Δ (gray diamonds) strain are compared. Error bars represent one SEM.