Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Jul 16;178(2):269–281. doi: 10.1083/jcb.200702141

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Localization of the Dam1 complexes at the microtubule plus end. (A) Schematic diagram of microtubule depolymerization and possible localization of the Dam1 complexes. (B) The Dam1 complexes accumulate at the end of a microtubule as it depolymerizes. (top) Representative time-lapse images of Dam1 complex localization during microtubule shrinkage. (bottom) Length of the microtubule (blue) and intensity of Dam1 and Ask1 GFP signals at the microtubule distal end (red). DAM1-4GFP ASK1-4GFP CFP-TUB1 P_GAL-CEN3-tetOs TetR-3CFP P_MET3-CDC20 cells (T4991) were treated as in Fig. 1 except that glucose-containing media were used throughout the experiment (i.e., CEN3 was continuously active). CFP (CEN3, tubulin; red) and GFP (the Dam1 complex; green) images were acquired every 10 s. AU, arbitrary unit. See Video 3. (C) The Dam1 complexes along a microtubule are collected at its plus end. T4991 cells were treated as in B. The Dam1 complex signal (Dam1-4GFP and Ask1-4GFP) at the microtubule plus end was quantified, and its fold increase during microtubule shrinkage was plotted against the extent of this shrinkage in the presence (pink) and absence (blue) of the Dam1 complexes along the microtubule. Fold increase was calculated by dividing the increase of the signal intensity (pre→post or post→post 2) by pre-signal intensity. Numbers in brown represent each microtubule in which the fold increase was measured consecutively (microtubule #3 is shown in the images). For more information, see supplemental note 31 (available at http://www.jcb.org/cgi/content/full/jcb.200702141/DC1).