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. 2007 Jul 16;178(2):297–308. doi: 10.1083/jcb.200701078

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Copyright © 2007, The Rockefeller University Press

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Figure 6. — Expression of PIPKIγY634F could not rescue EGF-stimulated cell migration in PIPKIγ668 knockdown HeLa cells. Parental HeLa cells or HeLa tet-off stable cell lines expressing HA-tagged wild-type PIPKIγ661 or PIPKIγY634F were transfected with control or PIPKIγ668 siRNA separately as indicated. (A) The expression of PIPKIγ in these cell lines was detected by anti-PIPKIγ antibody or anti-HA antibody. As the loading control, the amount of actin was detected by anti-actin antibody. (B) EGF was used to stimulate the migration of these cell lines. Quantifications are expressed as means ± SEM of three separate experiments (*, P < 0.01 compared with control siRNA–transfected HeLa cells).