Table II.
Cytoplasmic microtubule dynamics
| Cells | Growth rate | Shrinkage rate | Rescue frequency |
Catastrophe frequency |
Pause time | Dynamicity |
|---|---|---|---|---|---|---|
| μm/min | μm/min | events/s | events/s | % | dimers/s | |
| G1 | ||||||
| TUB2 | 1.41 ± 0.42 | 2.55 ± 0.76 | 0.0051 | 0.0074 | 8 | 40.9 |
| tub2-V169A | 0.54 ± 0.01 | 0.75 ± 0.36 | 0.0004 | 0.0013 | 83 | 2.3 |
| tub2-C354A | 0.63 ± 0.55 | 0.59 ± 0.26 | 0.0010 | 0.0015 | 79 | 2.8 |
| Preanaphase | ||||||
| TUB2 | 1.43 ± 0.68 | 1.78 ± 0.69 | 0.0063 | 0.0069 | 5 | 33.8 |
| tub2-V169A | 0.57 ± 0.24 | 0.75 ± 0.25 | 0.0017 | 0.0015 | 65 | 4.6 |
| tub2-C354A | 0.75 ± 0.74 | 0.98 ± 0.76 | 0.0010 | 0.0012 | 74 | 3.7 |
TUB2 (CUY1302): G1, n = 17 and t = 4,330 s; preanaphase, n = 10 and t = 4,070 s. tub2-V169A (CUY1844): G1, n = 8 and t = 4,500 s; preanaphase, n = 11 and t = 6,480 s. tub2-C354A (CUY1845): G1, n = 6 and t = 4,040 s; preanaphase, n = 7 and t = 4,200 s. n, number of time-lapse sequences; t, total length of time-lapse sequences obtained. Event rates are mean values ± SD.