Table I.
Defining the dependency among proteins for kinetochore assembly
| siRNA target |
Effects on kinetochore localizationa | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CENP-A | CENP-B | CENP-C | CENP-E | CENP-F | CENP-H | CENP-I | hMIS12 | Aurora B | MCAK | BUB1 | BUBR1 | MAD1 | MAD2 | hMPS1 | hZW10 | hROD | p150glued | HEC1 | Nuf2 | CDC20 | |
| CENP-A | + | + | + | + | + | ||||||||||||||||
| CENP-B | + | − | |||||||||||||||||||
| CENP-C | + | + | − | + | − | + | + | + | + | + | + | + | + | ||||||||
| CENP-H | − | + | + | − | |||||||||||||||||
| CENP-I | − | − | − | − | + | + | + | − | − | − | − | − | + | + | + | − | − | + | + | + | |
| hMIS12 | − | − | − | + | − | − | + | − | + | ||||||||||||
| Aurora B | − | − | − | − | + | + | − | + | − | − | − | − | − | − | |||||||
| hMPS1 | − | − | − | − | + | + | + | − | − | − | |||||||||||
| BUB1 | − | + | − | − | + | + | + | − | + | − | − | − | − | − | − | + | |||||
a
Kinetochore localization was examined by immunofluorescence and compared between control and target siRNA–transfected cells. + and – stand for the dependency or nondependency, respectively, between two proteins. Not all possible combinations were tested (blanks) because they were either already reported or because they can be inferred based on the assembly pathway.
bRefer to the main text for the complex hMis12–CENP-I relationship.