Table II.

Defects in kinetochore ultrastructure resulting from depletion of specific kinetochore proteins^a

siRNA	Kinetochores with triplate and >4 associated mts (normal)	Triplate with few (<4) or no associated mts	Partial plates and/or pulled out plates and/or fibrillar structure at outer plates	Fuzzy ball	Small kinetochore plates	Thin and/or punctate plates	Subjacent chromatin decondense and/or C-shaped plates	Total number of kinetochores counted
CENP-I		(no mts) 5	(<4 mt) 10	(no mts) 12				38
		(<4 mt) 3	(≥4 mts) 5	(ama) 1
			(ama) 2
NUF2	1	(no mts)( 2 (<4 mts) 6	(<4 mt) 27 (≥4 mts) 4	(no mts) 37 (<4 mts) 5 (ama) 1				83
NUF2	1	(no mts) 2	(<4 mt) 27	(no mts) 37				83
		(<4 mts) 6	(≥4 mts) 4	(<4 mts) 5
				(ama) 1
CENP-F	4		(<4 mt) 4	(ama) 3				12
			(≥4 mts) 1
CENP-C	2	(no mts) 4	(<4 mt) 5	(no mts) 1	(<4 mts) 4	(no mts) 7		50
		(<4 mts) 9	(≥4 mts) 1	(<4 mts) 5		(<4 mts) 1
			(ama) 2			(≥4 mts) 7
						(ama) 2
BUB1	9	(no mts) 2	(<4 mt) 1			(no mts) 6		22
		(<4 mts) 1	(ama) 1			(<4 mts) 1
						(ama) 1
hMis12	3	(no mts) 2	(<4 mt) 2				(no mts) 3	18
		(<4 mts) 1					(<4 mts) 5
							(ama) 2
Aurora B		(no mts) 7	(<4 mt) 3				(no mts) 3	67
		(<4 mts) 3	(ama) 3				(<4 mts) 24
							(≥4 mts) 10
							(ama) 14
Examples		Fig. 5 F, d	Fig. 2 G	Fig. 2, D–F	Fig. 5 F, a	Fig. 5 F, c and e	Fig. 7 F, a
			Fig. 5 F, b

^aMicrotubule (Mts) counts are based on a single section. A section with >4 microtubules is considered normal. ama, aberrant Mts association.