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. 2006 Nov 6;175(3):383–388. doi: 10.1083/jcb.200608031

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Copyright © 2006, The Rockefeller University Press

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Figure 1. — Cdo interacts with JLP. (A) Yeast transformed with the indicated vectors for Gal4 DNA-binding domain (BD) fused to the transmembrane (TM) plus intracellular regions (ICR) of Cdo or Necl-2 and Gal4 activation domain (AD) fused to a portion of JLP or Pals2 were plated on two-hybrid interaction-dependent selective medium. (B) Lysates from satellite cells of the indicated Cdo genotype were immunoprecipitated (IP) and Western blotted (WB) as indicated. (C and D) Lysates of C2C12 (C) or COS (D) cells transfected with S-tagged JLP derivative or control (−) expression vectors were pulled down with anti-S beads and blotted as indicated for Cdo or S epitope. Asterisks in D indicate S-JLP bands of the predicted size. Numbers above lanes correspond to the JLP amino acids in individual fragments, which are shown schematically in E. (E) S-JLP derivatives from D and the yeast two-hybrid clone (GAD-JLP) are shown to identify the Cdo-binding region of JLP. The p38α/β-binding region of JLP is also shown.