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. 2003 Oct 1;31(19):5501–5512. doi: 10.1093/nar/gkg753

Figure 4.

Figure 4

Ligase adenylation and Ku-IP6 binding. (a) Ligase adenylation. (i) Incorporation of [α-32P]ATP by a purified Ligase I titration. (ii) Effect of vanillin on Ligase I at 190 ng. A weak signal was generated, therefore (iii) shows effect of vanillin titration on 760 ng Ligase I. (iv) Incorporation of [α-32P]ATP by GM00558 extract. (v) Ten microgram extract pre-incubated (10 min at 4°C) with vanillin. (b) Ku binding to IP6. Effect of vanillin on Ku binding to 3H-labelled IP6. Counts per minute (c.p.m.) of labelled IP6 eluted plotted against vanillin concentration.