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. 2007 Aug 27;178(5):741–747. doi: 10.1083/jcb.200703034

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Copyright © 2007, The Rockefeller University Press

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Figure 2. — A feedback loop for Skp2 autoinduction. (A) A positive feedback loop that perpetuates Skp2 induction and Rb inactivation in the absence of exogenous mitogens. (B) RNA from serum-starved MEFs infected with Ad-LacZ or Ad-hSkp2 was analyzed by QPCR; the levels of Skp2, cyclin E1, and cyclin A mRNAs are plotted relative to their levels in the Ad-LacZ–infected cells. (C) Skp2 promoter activity in cells transfected with an irrelevant control siRNA or Skp2 siRNA, infected with Ad-LacZ or Ad-E7, and stimulated with 10% FCS for 24 h. The Western blot shows representative Skp2 and actin levels in MEFs transfected with control (Co) siRNA or Skp2 (S2) siRNA, serum starved, and stimulated with 10% FCS for 24 h. Error bars show mean ± SD.