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. 2007 Aug 27;178(5):757–764. doi: 10.1083/jcb.200704112

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — Disruption of mitochondrial morphology by Yme1 siRNA. Mitochondria were detected with Mitotracker. (A) Cells transfected with scrambled oligonucleotides for Yme1. (B) A cell 2 d after transfection with Drp1 siRNA. (C and D) Cells 2 d after transfection with Yme1 siRNA. (E and F) Cells 3 d after transfection with Yme1 siRNA. (G) Cells 2 d after transfection with Opa1 siRNA. (H) Cells 2 d after cotransfection with Opa1 and Yme1 siRNA (see Fig. S3 A for expression levels). (I and J) Cells transfected with Opa1 exon 4b– and 5b-specific siRNA (see Fig. S2 for quantification of the morphological defects; available at http://www.jcb.org/cgi/content/full/jcb.200704112/DC1). Bar, 10 μm.